KIU has its main campus at Kansanga, a location in Makindye Division in the south-eastern part of Kampala, Uganda's capital and largest city. The campus is approximately 7 kilometres (4.3 mi) south-east of Kampala's central business district, along the road to Ggaba. 

KIU's second campus is located in Ishaka in Bushenyi District, approximately 360 kilometres (220 mi), by road, south-west of Kampala.

KIU maintains a third campus, the KIU Dar es Salaam Constituent College, in Dar es Salaam, Tanzania.